Your qpcr assays not only rely on highly sensitive and specific detection but also a qpcr machine with fast cycling protocols and the right throughput for your needs.
Qiagen qpcr machine.
Together with optimized qiagen kits for real time pcr the rotor gene q enables streamlined analysis for a wide range of applications.
The reagent kits for qpcr and hrm are much easier to use with qiagen.
Cdna for sensitive and specific mirna detection.
Target nucleic acids can be quantified using either absolute quantification or relative quantification.
Rotor gene q from qiagen is indeed of a reasonable price for a reliable and outstanding performance in real time pcr.
You can fit a lot more samples per plate and use.
Qpcr assays arrays and kits.
Q rex software is operating and analysis software for life science qpcr applications.
A single cdna enables quantification of multiple mirnas.
Qiagen s real time pcr cycler the rotor gene q combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands.
Real time pcr thermal cyclers thermocyclers carry out quantitative pcr qpcr for experiments in gene expression genetic variation genotyping and specific detection of rare targets bacteria and viruses.
Also the light cycler is plate based while the rotor gene you can use tubes which makes it much more flexible.
I used to use a 96 well qpcr machine but 384 well qpcr machines are way better.
A choice of plug ins lets you add new functionality without affecting established workflows.
The mygo is.
A gradient function for assay optimization throughput and low noise are other important factors you need to consider when choosing a qpcr instrument.
Absolute quantification determines the absolute amount of target expressed as copy number or concentration whereas relative quantification determines as the first step of analysis the ratio between the amount of target and the amount of a control e g.
Video to demonstrate how to load strip tubes using a multichannel pipet for analysis on the rotor gene q real time pcr cycler.
Our block cyclers such as the qiaquant 96 and 384 for real time pcr and the qiaamplifier 96 for end point pcr deliver rapid reproducible results in broad a range of pcr applications.
